C&C – Controls & Calibrators
$1,000.00
Applies to every item below
Each item serves as a calibrator or standard in immunoassays and as an immunogen for antisera production. Purity is assessed by SDS-PAGE and concentration is determined spectrophotometrically, unless noted otherwise.
01 / Cardiac
Recombinant human cardiac Troponin I and C complex (cTnIC)
SourceExpressed in mammalian cell HEK293
Protein sequenceProduct of genes TNNI3 (UniProt Q6FGX2) and TNNC1 (UniProt P63316). His-tag at the C-terminus of each chain.
Purity> 95 %
ImmunoreactivityConfirmed by reaction with antibodies specific to different epitopes of the human cardiac TnI isoform
PurificationNi-NTA affinity, followed by size exclusion chromatography
StorageFrozen at −80 °C in 20 mM Tris, 1 mM TCEP, 150 mM NaCl, 1 mM CaCl₂, 1 mM KCl, at approximately 1 mg/mL
02 / Cardiac
Recombinant human NT-proBNP
SourceExpressed in mammalian cell HEK293
Protein sequenceUniProt P16860, positions 27–102. His-tag at the C-terminus.
Purity> 95 %
ImmunoreactivityConfirmed with antibodies specific to epitopes at positions 13–27, 27–31, and 42–46
PurificationNi-NTA affinity, followed by size exclusion chromatography
StorageFrozen at −80 °C in PBS
03 / Cardiac
Recombinant human NT-proBNP-huFc
SourceExpressed in mammalian cell HEK293
Protein sequenceUniProt P16860, positions 27–102. Human IgG1 Fc fragment at the C-terminus.
Purity> 95 %
ImmunoreactivityConfirmed with antibodies specific to epitopes at positions 13–27, 27–31, and 42–46
PurificationProtein A affinity, followed by size exclusion chromatography
StorageFrozen at −80 °C in PBS
04 / Haematology
Recombinant human fetal hemoglobin
SourceExpressed in E. coli
Protein sequenceProducts of genes HBA1 (UniProt P69905) and HBG2 (UniProt P69892). Supplied as a tetramer of two beta and two gamma chains.
Purity> 90 %
ConcentrationDetermined by the cyanmethemoglobin (Drabkin) method
PurificationIon exchange chromatography
StorageFrozen at −80 °C in 20 mM MES, 100 mM NaCl, pH 6.0
05 / Infectious disease
SARS-CoV-2 nucleocapsid protein N-terminal domain (Cov NTD)
SourceExpressed in mammalian cell HEK293
Protein sequenceUniProt P59595, positions 46–174. His-tag at the C-terminus.
Purity> 95 %
ImmunoreactivityConfirmed by reaction with antibodies specific to various epitopes
PurificationNi-NTA affinity, followed by size exclusion chromatography
StorageFrozen at −80 °C in PBS
06 / Haematology · monoclonal antibody
Monoclonal antibody 30D12 against red blood cells (Glycophorin A)
This monoclonal antibody specifically recognizes human glycophorin A and mediates erythrocyte agglutination through cross-linking of red blood cells.
SourceMouse antibody expressed by hybridoma cells
Antigen targetGlycophorin A on human red blood cells
Purity> 95 %
PurificationAffinity purification
StorageFrozen at −80 °C in PBS
